primary antibodies fth1  (Cell Signaling Technology Inc)

Journal: FEBS Open Bio

Article Title: Polydatin ameliorates ovalbumin‐induced asthma in a rat model through NCOA4 ‐mediated ferroautophagy and ferroptosis pathway

doi: 10.1002/2211-5463.70090

Figure Lengend Snippet: Effects of polydatin on the levels of ferroautophagy‐related indicators in asthmatic rats. (A) Interaction between NCOA4 and FTH1 in rats between groups. (B, C) The expression of NCOA4 and FTH1 in lung tissue of rats in each group ( n = 6). Scale bar = 50 μm. (D–G) The mRNA expression of NCOA4, FTH1, Beclin1, and P62 in lung tissues of rats among different groups ( n = 10). (H) Western blotting bands of NCOA4, FTH1, Beclin1, and P62 in lung tissue of rats among different groups. (I–L) The protein expression of NCOA4, FTH1, Beclin1, and P62 in lung tissues of rats among different groups ( n = 3). 1, control group; 2, model group; 3, polydatin group; 4, 3‐MA group; 5, Fer‐1 group; * indicates compared to the control group, P < 0.05; # indicates compared to the model group, P < 0.05. Data were presented as mean ± SD. The statistical testing methods used are SNK‐q or Dunnett's T3.

Article Snippet: The sections were then incubated with the nuclear receptor coactivator 4 (NCOA4) primary antibody (1 : 50, A5695; ABclonal, Wuhan, China) and the ferritin heavy chain 1 (FTH1) primary antibody (1 : 200, 4393; Cell Signaling Technology, Massachusetts, USA) at 25 °C for 1.5 h, followed by a rinse using PBS (three 5‐min washes).

Techniques: Expressing, Western Blot, Control

Journal: PLOS ONE

Article Title: (−)-Epicatechin regulates endoplasmic reticulum stress and promotes ferroptosis in lung cancer cells via the PERK/eIF2α/ATF4 signaling pathway

doi: 10.1371/journal.pone.0313010

Figure Lengend Snippet: A: Representative external images of the tumor-bearing nude mice on the 28 th day. B: Tumor size. C: Tumor volume. D: Tumor weight. E: Immunohistochemical detection of Ki-67 expression. F: Detection of Fe 2+ content using a reagent kit. G: Western blotting was performed to detect the expression levels of the ferroptosis-related proteins SLC7A11, GPX4, and FTH1. *** P<0.001.

Article Snippet: Primary antibodies against SLC7A11 (1:2000, ab175186, Abcam, UK), GPX4 (1:1000, ab125066, Abcam, UK), FTH1 (1:1000, PA5-27500, Thermo Fisher Scientific, USA), p-PERK (1:1000, PA5-40294, Thermo Fisher Scientific, USA), PERK (1:1000, PA5-99447, Thermo Fisher Scientific, USA), p-eIF2α (1:1000, MA5-15133, Thermo Fisher Scientific, USA), eIF2α (1:1000, AHO0802, Thermo Fisher Scientific, USA), ATF4 (1:1000, ab1371, Abcam, UK), CHOP (1:1000, MA1-250, Thermo Fisher Scientific, USA), and β-actin (1:1000, ab8226, Abcam, UK) were added overnight at 4 °C.

Techniques: Immunohistochemical staining, Expressing, Western Blot

Journal: PLOS ONE

Article Title: (−)-Epicatechin regulates endoplasmic reticulum stress and promotes ferroptosis in lung cancer cells via the PERK/eIF2α/ATF4 signaling pathway

doi: 10.1371/journal.pone.0313010

Figure Lengend Snippet: A: CCK-8 detection of lung cancer cell viability. B: CCK-8 detection of BEAS-2B cell viability. C: Colony formation assay for detecting lung cancer cell proliferation. D: Transwell assay for detecting lung cancer cell migration. E: Transwell assay for detecting lung cancer cell invasion. F: Detection of Fe 2+ content in lung cancer cells using a reagent kit. G: Western blotting was used to detect the expression levels of the ferroptosis-related proteins SLC7A11, GPX4, and FTH1 in lung cancer cells. ** P<0.01, * * * P<0.001.

Article Snippet: Primary antibodies against SLC7A11 (1:2000, ab175186, Abcam, UK), GPX4 (1:1000, ab125066, Abcam, UK), FTH1 (1:1000, PA5-27500, Thermo Fisher Scientific, USA), p-PERK (1:1000, PA5-40294, Thermo Fisher Scientific, USA), PERK (1:1000, PA5-99447, Thermo Fisher Scientific, USA), p-eIF2α (1:1000, MA5-15133, Thermo Fisher Scientific, USA), eIF2α (1:1000, AHO0802, Thermo Fisher Scientific, USA), ATF4 (1:1000, ab1371, Abcam, UK), CHOP (1:1000, MA1-250, Thermo Fisher Scientific, USA), and β-actin (1:1000, ab8226, Abcam, UK) were added overnight at 4 °C.

Techniques: CCK-8 Assay, Colony Assay, Transwell Assay, Migration, Western Blot, Expressing

Journal: PLOS ONE

Article Title: (−)-Epicatechin regulates endoplasmic reticulum stress and promotes ferroptosis in lung cancer cells via the PERK/eIF2α/ATF4 signaling pathway

doi: 10.1371/journal.pone.0313010

Figure Lengend Snippet: A: CCK-8 detection of lung cancer cell viability. B: Detection of Fe 2+ contents in lung cancer cells using a reagent kit. C: Western blotting was used to detect the expression levels of the ferroptosis-related proteins SLC7A11, GPX4, and FTH1 in lung cancer cells. *** P<0.001 vs. NC# P<0.05, # # P<0.01, # # # P<0001 vs. EC.

Article Snippet: Primary antibodies against SLC7A11 (1:2000, ab175186, Abcam, UK), GPX4 (1:1000, ab125066, Abcam, UK), FTH1 (1:1000, PA5-27500, Thermo Fisher Scientific, USA), p-PERK (1:1000, PA5-40294, Thermo Fisher Scientific, USA), PERK (1:1000, PA5-99447, Thermo Fisher Scientific, USA), p-eIF2α (1:1000, MA5-15133, Thermo Fisher Scientific, USA), eIF2α (1:1000, AHO0802, Thermo Fisher Scientific, USA), ATF4 (1:1000, ab1371, Abcam, UK), CHOP (1:1000, MA1-250, Thermo Fisher Scientific, USA), and β-actin (1:1000, ab8226, Abcam, UK) were added overnight at 4 °C.

Techniques: CCK-8 Assay, Western Blot, Expressing

Journal: PLOS ONE

Article Title: (−)-Epicatechin regulates endoplasmic reticulum stress and promotes ferroptosis in lung cancer cells via the PERK/eIF2α/ATF4 signaling pathway

doi: 10.1371/journal.pone.0313010

Figure Lengend Snippet: A: CCK-8 detection of lung cancer cell viability. B: Detection of Fe 2+ contents in lung cancer cells using a reagent kit. C: Western blotting was used to detect the expression levels of the ferroptosis-related proteins SLC7A11, GPX4, and FTH1 in lung cancer cells. ** P<0.01, * * * P<0.001 vs. NC# P<0.05, # # P<0.01, # # # P<0001 vs. EC.

Article Snippet: Primary antibodies against SLC7A11 (1:2000, ab175186, Abcam, UK), GPX4 (1:1000, ab125066, Abcam, UK), FTH1 (1:1000, PA5-27500, Thermo Fisher Scientific, USA), p-PERK (1:1000, PA5-40294, Thermo Fisher Scientific, USA), PERK (1:1000, PA5-99447, Thermo Fisher Scientific, USA), p-eIF2α (1:1000, MA5-15133, Thermo Fisher Scientific, USA), eIF2α (1:1000, AHO0802, Thermo Fisher Scientific, USA), ATF4 (1:1000, ab1371, Abcam, UK), CHOP (1:1000, MA1-250, Thermo Fisher Scientific, USA), and β-actin (1:1000, ab8226, Abcam, UK) were added overnight at 4 °C.

Techniques: CCK-8 Assay, Western Blot, Expressing

Journal: PLOS ONE

Article Title: (−)-Epicatechin regulates endoplasmic reticulum stress and promotes ferroptosis in lung cancer cells via the PERK/eIF2α/ATF4 signaling pathway

doi: 10.1371/journal.pone.0313010

Figure Lengend Snippet: A: Western blotting was used to detect the expression levels of PERK/eIF2α/ATF4 signaling pathway-related proteins and CHOP in lung cancer cells. B: Flow cytometry was used to measure the Ca 2+ levels in lung cancer cells. C: CCK-8 detection of lung cancer cell viability. D: Detection of Fe 2+ content in lung cancer cells using a reagent kit. E: Western blotting was used to detect the expression levels of the ferroptosis-related proteins SLC7A11, GPX4, and FTH1 in lung cancer cells. ** P<0.01, * * * P<0.001 vs. NC# P<0.05, # # P<0.01, # # # P<0001 vs. EC.

Article Snippet: Primary antibodies against SLC7A11 (1:2000, ab175186, Abcam, UK), GPX4 (1:1000, ab125066, Abcam, UK), FTH1 (1:1000, PA5-27500, Thermo Fisher Scientific, USA), p-PERK (1:1000, PA5-40294, Thermo Fisher Scientific, USA), PERK (1:1000, PA5-99447, Thermo Fisher Scientific, USA), p-eIF2α (1:1000, MA5-15133, Thermo Fisher Scientific, USA), eIF2α (1:1000, AHO0802, Thermo Fisher Scientific, USA), ATF4 (1:1000, ab1371, Abcam, UK), CHOP (1:1000, MA1-250, Thermo Fisher Scientific, USA), and β-actin (1:1000, ab8226, Abcam, UK) were added overnight at 4 °C.

Techniques: Western Blot, Expressing, Flow Cytometry, CCK-8 Assay